FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123942384
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG326150.056.69147546
CGTATGC340500.054.68877847
CTCGTAT344900.052.60594645
TATGCCG363100.051.1461149
TATCTCG367650.048.41271642
GATCGGA658750.045.511391
GACCGTA428800.044.1044332
AATGCCG746450.040.89943324
ACCGTAG466950.040.23142233
AGCGGTT760000.040.13117610
TCGGAAG800400.039.4291733
ATGCCGT471050.038.95789750
GCTTATC471600.038.03412639
AAGAGCG823300.038.021687
GTATGCC498300.037.5255948
CGTAGCT516300.036.28375635
GCCGTCT513700.035.75634452
TGCCGAG865000.034.64867426
TAGCTTA538850.034.19148337
GAGCGGT910500.033.729259
Produced by FastQC (version 0.11.5)