FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123942384
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1288960.10399670866424517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATT233400.021.1945321
TAGCATA548050.018.7658928
TCCGATC128800.017.73072224
GTAGCAT592050.017.528517
CATATGC610550.017.06367111
GCATATG610300.017.04296910
GCCAGTA666750.015.76033
CAGTAGC693700.015.0732235
GCGGTGT461350.015.0518121
GATCGGA175000.014.9616411
GTACGCA695150.014.7694249
TACGCAC692200.014.7627950
ATATGCT719400.014.73117412
AGTACGC696600.014.69387848
CCAGTAG715600.014.6068524
TGAACCG306950.014.5991813
CGTATCA85000.014.44907749
TATGCTT747250.014.11752213
ACGCACG734350.014.04390151
AAGTACG736200.013.91219747
Produced by FastQC (version 0.11.5)