FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116473786
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5782710.4964816718501792Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACCGGCAT2856260.24522771158138534Illumina Paired End PCR Primer 2 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1195450.064.79088646
CTCGTAT1194850.063.77711545
CGTATGC1214100.063.68023747
TATGCCG1227600.062.8872349
ATGCCGT1329550.057.12733550
GTATGCC1374250.056.27925548
GCCGTCT1352500.056.07512352
CATCTCG1337000.054.8505942
TGCCGTC1389400.054.7965951
TCTCGTA1350950.053.73047344
ATCTCGT1385100.052.3164543
CCGTCTT1515250.050.38582653
GATCGGA2483400.049.9202881
AATGCCG2531950.048.43845424
AGCGGTT2538650.048.41877710
TCGGAAG2627900.047.9595383
AAGAGCG2614800.047.6400037
CGTCTTC1614750.047.54326254
TGCCGAG2608150.046.611426
GAGCGGT2643900.046.529
Produced by FastQC (version 0.11.5)