FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139319879
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCCACGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2433090.17464054788620653Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG645850.060.8821346
CGTATGC667800.059.3794547
TATGCCG674700.058.5152749
CTCGTAT656250.058.22052845
GATCGGA1102000.050.3799441
ATGCCGT792150.049.16372350
AATGCCG1166350.047.36887724
GTATGCC841200.047.1179148
AGCGGTT1187650.046.3053510
GCCGTCT854700.045.68839652
TCGGAAG1276300.045.0131533
AAGAGCG1259450.044.6326187
TGCCGTC889500.044.10893651
AGACCGC957900.043.8604331
TCTCGTA811350.043.4016244
ACGCATC826450.043.0394739
CGCATCT824000.042.7013240
GAGCGGT1281650.042.4632459
CATCTCG836250.042.43334642
TGCCGAG1283900.042.30360826
Produced by FastQC (version 0.11.5)