EGAF00005732596 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005732596
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	96699036
Sequences flagged as poor quality	0
Sequence length	75
%GC	52

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	2304112	2.382766256325451	Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATAT	1290880	1.3349460898451977	Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATA	323049	0.3340767533608091	Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAC	148999	0.15408530029192846	Illumina Paired End PCR Primer 2 (97% over 36bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATAT	140892	0.1457015559079617	Illumina Paired End PCR Primer 2 (97% over 37bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAACA	127012	0.13134774166724889	Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAG	122569	0.12675307331915905	Illumina Paired End PCR Primer 2 (97% over 36bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG	114428	0.11833416829512136	No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGCCATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAGA	103515	0.10704863696883182	Illumina Paired End PCR Primer 2 (97% over 36bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	442690	66.903465	46
CTCGTAT	439030	66.71149	45
CGTATGC	443310	66.66606	47
TATCTCG	433450	66.48332	42
TATGCCG	443830	66.36129	49
ATGCCGT	447710	65.25754	50
GTATGCC	453685	64.96375	48
GCCGTCT	449890	64.82611	52
TCTCGTA	444185	64.78685	44
TGCCGTC	452295	64.63596	51
ATATCTC	440565	64.616554	41
ATCTCGT	447440	64.09917	43
CATATCT	444710	63.98654	40
CCGTCTT	463805	62.92476	53
GCTTGAA	447965	61.945744	62
CGTCTTC	476605	61.46638	54
TGCTTGA	465970	61.17064	61
CTTGAAA	447160	61.09675	63
CCATATC	462215	60.95677	39
CTGCTTG	496065	59.94157	60