EGAF00005732598 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005732598
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	130912665
Sequences flagged as poor quality	0
Sequence length	75
%GC	51

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGATAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	464917	0.35513523462378527	Illumina Paired End PCR Primer 2 (97% over 37bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG	267802	0.20456538716097486	No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT	188411	0.14392114009748408	No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGACGATAAT	169279	0.1293068168767323	Illumina Paired End PCR Primer 2 (97% over 38bp)
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATG	155291	0.11862183082133422	No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCAT	131907	0.10075954072128927	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	93010	64.093185	46
CGTATGC	93755	63.719948	47
TATGCCG	94820	62.773773	49
CTCGTAT	90970	62.41789	45
GTATGCC	108205	55.1561	48
GCCGTCT	106735	54.949963	52
ATGCCGT	108880	54.17895	50
TCTCGTA	104420	53.192642	44
TGCCGTC	113045	52.078087	51
GATCGGA	164530	50.905514	1
GATAATC	110035	50.70013	39
AATGCCG	171630	49.460304	24
ATCTCGT	113750	48.92418	43
CGATAAT	120080	48.700634	38
AGCGGTT	174635	48.684853	10
TCGGAAG	177330	48.655674	3
CGACGAT	123740	48.191944	35
AAGAGCG	179715	47.885124	7
CGGTTCA	179255	47.367413	12
ACCGACG	128850	47.36299	33