FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130912665
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG2404560.1836766519114098No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1764760.13480437511527246No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATG1330680.10164639150841517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATT354500.037.2323571
TGAACCG338300.030.22933213
CGTATCA113900.025.71187649
GATCGGA258700.025.3298281
TAGCATA834900.022.3505048
CATATGC875050.021.49058711
CGCCGTA143100.021.42880846
GCATATG878050.021.42110610
GTAGCAT890500.021.0705577
GCCAGTA921000.020.3190233
TCGGAAG326650.019.9665013
TCCGATC148750.019.52727524
ATCGGGA230600.019.0344582
CAGTAGC1014700.018.5697355
ATATGCT1041600.018.46436312
GCGGTGT600050.018.3887331
TATGCTT1032850.018.33900613
ATGCTTG1064600.017.78637714
GCCGTAT195250.017.41893647
AACCGCT243400.017.22980715
Produced by FastQC (version 0.11.5)