FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100299345
Sequences flagged as poor quality0
Sequence length75
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA3841050.38295863248159795Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAATTAT1069100.10659092539437819Illumina Paired End PCR Primer 2 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG672250.063.086346
CGTATGC681750.062.14689647
CTCGTAT675700.061.31891345
TATGCCG696700.060.8170349
TATCTCG686400.059.65182542
ATGCCGT786200.053.70941550
GTATGCC807300.052.55774748
TCTCGTA779300.052.5552744
GATCGGA1082750.050.948211
GCCGTCT829100.050.73112552
ATCTCGT830300.049.39763643
AATGCCG1154800.048.4682224
AGCGGTT1150250.048.2757510
TGCCGTC879250.048.05376451
CGATAAT874850.047.8864335
ACCGATA896200.046.83403833
CCGATAA895950.046.70588734
TTATCTC879150.046.64444441
TCGGAAG1216100.046.632073
AAGAGCG1216650.046.2491727
Produced by FastQC (version 0.11.5)