FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127298732
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA5523440.43389591657519416Illumina Paired End PCR Primer 2 (97% over 39bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATCCTAAT1513410.11888649448605663Illumina Paired End PCR Primer 2 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG994600.063.82861746
CGTATGC1015150.062.61128247
TATGCCG1020700.062.0004449
CTCGTAT981950.061.9558845
ATGCCGT1103450.056.58561750
GTATGCC1176650.053.97657448
GCCGTCT1162800.053.708252
CCTAATC1121350.053.26692639
GATCGGA1618050.052.1564981
TCTCGTA1141850.051.87848344
TGCCGTC1212050.051.56363751
CTAATCT1157150.051.4877440
AATGCCG1697700.050.0064524
AGCGGTT1705150.049.9518310
ATCTCGT1186200.049.90118843
AAGAGCG1794650.048.2545437
TCGGAAG1794600.048.2135963
CCGTCTT1302450.048.03505753
GAGCGGT1799250.047.479339
CGGTTCA1815900.046.81413712
Produced by FastQC (version 0.11.5)