EGAF00005732610 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005732610
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	172411620
Sequences flagged as poor quality	0
Sequence length	75
%GC	50

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACTCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	501422	0.2908284256014763	Illumina Paired End PCR Primer 2 (97% over 36bp)
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG	330532	0.19171097632514558	No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCACTCAAT	211491	0.122666326086374	Illumina Paired End PCR Primer 2 (97% over 37bp)
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT	190456	0.11046587231185463	No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATG	181353	0.1051860657651729	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	97430	62.51105	46
CGTATGC	98925	61.74435	47
CTCGTAT	95340	60.4839	45
TATGCCG	100960	60.33256	49
ATGCCGT	111625	53.906765	50
GTATGCC	115925	52.683983	48
GCCGTCT	120605	49.842667	52
TGCCGTC	122785	49.117634	51
GATCGGA	191730	48.928524	1
TCTCGTA	115810	48.150543	44
AATGCCG	197220	47.100067	24
AGCGGTT	201620	46.4691	10
TCGGAAG	209550	45.657097	3
ATCTCGT	124085	44.97754	43
AAGAGCG	212355	44.764874	7
CGGTTCA	213000	43.81652	12
TGCCGAG	211690	43.250977	26
AGAGCGG	222045	43.075436	8
CGGAAGA	225575	42.33407	4
GAGCGGT	223235	41.839878	9