FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732615

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732615
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100829665
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG1081270.10723728974007798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCATT241750.030.6191441
GATCGGA230600.026.6798361
CGTATCA99300.026.30008549
TCCGATC125700.022.61404824
TGAACCG288950.022.34669513
CGCCGTA126500.022.1147346
AACCGCT201800.020.92196515
GCGTCGT128100.019.95415311
TAGCATA468700.018.7245778
ATCGGGA217650.018.2738592
TCGGAAG309000.018.1232193
AGAGCGT200050.018.0722648
GCATATG504900.017.66879710
GTAGCAT506000.017.6170927
CATATGC506900.017.4689911
GTACGCA505750.017.21043849
TACGCAC506300.017.15824150
AAGAGCG287950.017.1081627
AGTACGC512150.017.02134348
GCCGTAT189400.016.95632647
Produced by FastQC (version 0.11.5)