FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732616

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732616
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128356350
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA6246230.48663194302424456Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGGCCAATAT1467880.11435974924497308Illumina Paired End PCR Primer 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1206650.064.4174446
CGTATGC1219800.064.1156147
TATGCCG1229350.063.40994349
CTCGTAT1211200.063.084545
TATCTCG1191050.062.3727242
ATGCCGT1330600.057.87327250
GTATGCC1377050.056.7890848
TCTCGTA1314950.056.29644844
GCCGTCT1381200.055.6085952
GATCGGA1866100.054.952321
TGCCGTC1408700.054.5983551
ATCTCGT1363650.054.1681843
AGCGGTT1935500.052.6909910
AATGCCG1939150.052.6612524
ATATCTC1426950.052.226141
TCGGAAG2027550.051.6104973
AAGAGCG2032550.051.076247
CGGTTCA2036700.050.0864212
CAATATC1493800.049.96056739
CCGTCTT1545500.049.85870753
Produced by FastQC (version 0.11.5)