FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005732618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146063552
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG3010530.20611096736850545No Hit
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGAAAGCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA2787390.19083405557602762Illumina Paired End PCR Primer 2 (97% over 37bp)
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATG1914630.1310819827248895No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1812260.12407338964343412No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCAT1469440.10060278419081578No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)