FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146063552
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG2738450.187483459254777No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1752290.11996764257793757No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATG1638370.11216829781053113No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCAT1467070.1004405260526596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA179900.036.1694449
CGCCGTA198450.035.77615446
GATCGGA365650.032.5416151
CGGCATT281750.029.2732811
GCGTCGT227900.028.14877511
GCCGTAT276350.026.55447247
TCCGATC181900.026.49361624
CGTGTAG289000.024.68103415
TCGGAAG470500.024.283623
GTCGTGT249000.024.25359213
AAGAGCG435750.023.7138397
AGAGCGT345650.023.6497488
TGAACCG334150.023.31653413
TAGCATA898000.022.9150948
TCGTGTA242550.022.53920214
CCGTATC315800.022.30882548
GTAGATC327600.021.5929431
GTAGCAT973750.021.322917
GCATATG1014950.020.47342510
CATATGC1013900.020.44420611
Produced by FastQC (version 0.11.5)