FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142824811
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG3351370.23464900646709064No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT2385240.16700459698140263No Hit
GCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATG2035400.1425102533480685No Hit
GGCGGTGTGTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCAT1836730.12860020518423793No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGAACCAT1572400.11009291655915442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA263200.046.7182549
CGCCGTA290450.044.45757346
CGGCATT504900.044.3728181
TCCGATC257400.038.59776324
TGAACCG546800.037.05508813
GATCGGA596600.036.6157341
AACCGCT373100.034.66818215
GCGTCGT322800.032.77539411
CGTGTAG392850.032.2574715
TCGGAAG680400.032.2175873
AGAGCGT436900.031.639588
AAGAGCG607100.031.0639347
GCCGTAT435650.030.92308247
ATCGGGA305250.030.470132
TCGTGTA319650.030.14954614
GTCGTGT345350.029.69486813
CCGATCA260050.029.15697325
GTATCAT427150.028.90783150
GTAGATC461100.028.90364531
GGTCGCC529750.028.2752943
Produced by FastQC (version 0.11.5)