EGAF00005732628 FastQC Report

Basic Statistics

Measure	Value
Filename	EGAF00005732628
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	165442234
Sequences flagged as poor quality	0
Sequence length	75
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTATACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA	2006047	1.2125362136974045	Illumina Paired End PCR Primer 2 (97% over 38bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGCTATACAT	233205	0.14095856563445583	Illumina Paired End PCR Primer 2 (97% over 39bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
TCGTATG	276420	64.80279	46
CGTATGC	277565	64.57401	47
CTCGTAT	272925	64.18729	45
TATGCCG	279370	64.13127	49
ATGCCGT	292885	61.156277	50
TCTCGTA	277085	60.39559	44
GCCGTCT	297165	60.03289	52
GTATGCC	299570	59.86693	48
ATCTCGT	283065	59.11855	43
TGCCGTC	303290	59.118454	51
CATCTCG	285365	58.778088	42
CCGTCTT	310905	57.04597	53
ATACATC	297115	56.49091	39
GATCGGA	374395	55.121227	1
CGTCTTC	321390	55.093765	54
AGCGGTT	415250	54.14191	10
CGCTATA	334950	53.858303	35
AATGCCG	414955	53.75672	24
TACATCT	314200	53.480648	40
TCGGAAG	423505	53.286274	3