FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165442234
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA2961000.17897485596090296Illumina Single End PCR Primer 1 (100% over 57bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA2644850.064.6935949
CGCCGTA2675050.064.0366946
GCCGTAT2729900.062.86598247
CCGTATC2762600.061.87551548
TGGTCGC2807800.061.44869242
GGTCGCC2865900.060.21499643
TCGCCGT2874800.059.54520445
GTGGTCG2921000.059.39647741
GCGTCGT3025250.058.33252711
GTATCAT2946700.058.32979250
GATCGGA3042000.058.2554631
TCGTGTA3071100.057.13387714
GTCGTGT3094650.057.0600313
CGTGTAG3113050.056.4879615
AGAGCGT3153750.056.335798
CGTCGTG3158000.055.93287712
CGGTGGT3139900.055.7384239
TCGGAAG3232450.055.249683
GTCGCCG3156700.055.0285544
AAGAGCG3243450.054.6275837
Produced by FastQC (version 0.11.5)