FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences241225468
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGATAATTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA20341070.8432389070958295Illumina Paired End PCR Primer 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2676900.063.18166446
CGTATGC2685050.062.9159947
TATGCCG2684750.062.279849
CTCGTAT2709600.061.87082345
TATCTCG2659500.061.8459842
GATCGGA2820350.060.793121
CGATAAT2821250.059.8263935
ACCGATA2851150.059.29829833
CCGATAA2854850.059.0916234
AATGCCG3146150.057.16625624
ATGCCGT2929800.057.07313550
AGCGGTT3173350.057.04149610
TCTCGTA2913950.056.62235344
GTATGCC3014200.056.07340648
TCGGAAG3258900.055.5369573
AAGAGCG3246000.055.5327267
ATCTCGT2981050.055.36536443
GAGCGGT3291200.055.043299
AGACCGA3082000.054.95915231
GCCGTCT3050500.054.76927652
Produced by FastQC (version 0.11.5)