FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences98982180
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA14403541.4551649599958294Illumina Paired End PCR Primer 2 (97% over 36bp)
GATCGGAAGAGCGGTTCAGCAGGAATGCCGAGATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACCAAT1263840.12768358910664526Illumina Paired End PCR Primer 2 (97% over 37bp)
TATCGGAAGAGCGGTTCAGCAGGAATGCCGAGACCGTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1232920.12455979450038379Illumina Paired End PCR Primer 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2221550.064.4442646
CGTATGC2227150.064.435747
CTCGTAT2049200.064.0641945
TATGCCG2239000.063.9666549
ATGCCGT2291100.062.08945550
GTATGCC2343600.061.1355248
GCCGTCT2314850.060.97775752
TGCCGTC2348000.060.5700351
TCTCGTA2071450.060.55528644
ATCTCGT2098300.059.7357443
CCGTCTT2383350.058.58877653
GATCGGA2685250.058.3922841
CGTCTTC2424800.057.34541354
ACCAATC2139850.057.33823439
AGCGGTT2974600.057.02986510
AATCTCG2164050.057.0234442
AATGCCG2974350.056.5746724
TCGGAAG3041800.056.438553
AAGAGCG3040300.056.305337
CCAATCT2178700.056.24735640
Produced by FastQC (version 0.11.5)