FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134328718
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCCAGTTACCTTGTTTTTTTTTGGTCTTTTTGTTATTTTGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3292100.2450778991280182No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATA1923070.14316149432766864No Hit
GCCAGTTACCTTGTTTTTTTTTGGTCTTTTTGTTATTTTGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550240.11540644644580023No Hit
GTGCCAGTTACCTTGTTTTTTTTTTGGTCTTTTTGTTATTTTGTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1476580.10992288335544154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCCAG2747750.033.5820581
TGCCAGT3091650.030.3047852
TCAGTTA714850.028.0636392
TATGCCG74300.027.53212449
CCAGTTA3695800.026.694624
CGGTTAC191750.026.6906033
AGTTACC4309850.025.8744166
CAGTTAC4184900.025.072125
GTTACCT4797850.024.027617
TACCTTG4858600.023.7349859
GCCAGTT4240000.022.907253
ACCTTGT5096150.022.65974210
TTACCTT5243200.022.3774438
GTGCTAG293050.021.9858111
CCTTGTT5400750.021.45198411
GTCAGTT1001600.020.5092471
CTTGTTT5706100.020.37474312
GCCGGTT291900.016.9332471
TGTTTTT7110750.016.42802214
GATCGGA143800.016.3451291
Produced by FastQC (version 0.11.5)