FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134328718
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG3649400.2716768278842652No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT2022060.15053073014513546No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1568530.11676803168775868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATTTA606600.025.8770941
TTAGCTC1458850.025.1541373
ATTTAGC2193350.024.5474111
GTTCGGT894350.024.51906648
GTACGGT312250.023.65241848
ACGGTCC1305600.023.63101450
GCATATC368050.023.4606786
GGGTACG421800.023.21103146
CAGCGCG1501950.022.91106458
GATCGGT711300.022.89124348
GATTTAG743400.022.857051
GCCAGTA1249600.022.6734543
GAACGGT1063800.022.66705148
GGTTCGG1113850.022.53074847
CCAGTAG1267200.022.4225884
GGTCCTC2793550.022.27996852
CGCGGGA1453750.022.02799261
AACGGTC1074550.022.0256149
GCCGTAT89650.021.94184147
TAGAGCG848700.021.45667515
Produced by FastQC (version 0.11.5)