FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123624931
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATA2111180.1707729972362937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG101700.020.66500749
AGTTACC445850.019.237726
GATCGGA137800.016.7265821
TCGTATG129450.016.62923646
GTTACCT543000.016.4307147
TCAACGC1043300.015.76239522
ATCAACG1044800.015.73289221
GGGGAAT1077000.015.37166268
CAACGCA1077800.015.27076623
AACGCAA1082200.015.20900324
ACGCAAG1095850.015.03995325
GGACTTA1094750.014.97679414
GTACAAA1120150.014.804891
TACCTTG609250.014.6610089
CGCAAGC1125050.014.6190226
GACTTAA1154150.014.28962715
ACTTAAT1174350.014.09373616
CAGTTAC589100.013.9160125
TATGACC1184100.013.82452834
TAATCAA1200100.013.82327219
Produced by FastQC (version 0.11.5)