FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123624931
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTG3953140.31976883368290815No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACT1887620.15268926621281592No Hit
GCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1756610.14209188921609994No Hit
TGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGC1367390.11060794848896618No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCATA1304550.023.7251558
CCAGTAG1310400.023.6846084
GCCAGTA1383900.022.5211453
GTAGCAT1407000.022.229617
GCATATG1409850.022.01905810
CATATGC1467300.021.27221511
ATATGCT1508000.020.76388512
CAGTAGC1512700.020.6936595
TATGCTT1554550.020.11442413
ATGCTTG1557550.020.0428814
TGCCAGT1594250.019.785342
AGTAGCA1626900.019.3196166
TACGCAC1646750.018.80992950
GCTTGTC1659650.018.79767216
ACGCACG1647800.018.78020151
TGCTTGT1679950.018.66719415
CGTATCA115700.018.5430949
AGTACGC1678600.018.50343348
CCGGTAC1675800.018.49253859
TTAAGCC1694000.018.37923430
Produced by FastQC (version 0.11.5)