FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005732644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157937118
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATA4034500.2554497670395632No Hit
ACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCATGGGGAATAAT2012610.12743109570987612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG144050.034.64544349
TCGTATG152600.032.7983346
CTCGTAT188650.026.146345
CGTATGC194400.025.9753947
CCGAAAG226550.022.58136434
AGACCGA237100.021.68746431
GATCGGA255700.021.509671
GACCGAA254300.020.17992832
GTATGCC269600.018.97300548
GAGACCG276000.018.64437530
GTACAAA1651050.018.3280281
ACCGAAA284700.018.24475133
AATGCCG318450.017.6369924
GGGGAAT1735000.017.4949168
CCGAGAC297950.017.4103528
CGAGACC300100.017.2735329
ATCAACG1784800.016.92298321
TACAAAG1806650.016.7717292
TCAACGC1811200.016.68964622
CAACGCA1833600.016.4919723
Produced by FastQC (version 0.11.5)