FastQCFastQC Report
Thu 11 Aug 2022
EGAF00005732843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005732843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50965864
Sequences flagged as poor quality0
Sequence length150
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT1943820.38139645783303117No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCG1899900.3727789251252564No Hit
GGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCG1659340.3255787049936012No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT1646230.32300639502550177No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT1612920.3164706478830615No Hit
CTCGGCCCCGGTCGCGGTCCCCCGTCCCGGGCGGGGGCGGGCGCGCCGGC1364490.26772625693150226No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGC1215220.23843802589121219No Hit
GGGGCCTCGGCCCCGGTCGCGGTCCCCCGTCCCGGGCGGGGGCGGGCGCG1154980.22661834988218782No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1055880.20717396255658496No Hit
GCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGC1037720.20361079329490028No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT1010240.19821894905970786No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG985560.19337649215561223No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG950430.18648364324795907No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG867840.17027867907821595No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC843040.1654126770027876No Hit
GGCGGCTCCGCCGCCGCGTCCTCGGACCCGTCCCCCCGACCTCCGCGGGG831420.1631327195787361No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT827710.16240478136503286No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGAT820620.16101365415879146No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT819470.16078801293351958No Hit
GCGGCGGGGAGCGGTCCCCGGCCGCGGCCCCGACGGCGTGGGTGTCGGCG789160.15484089507439724No Hit
GCCTCGGCCCCGGTCGCGGTCCCCCGTCCCGGGCGGGGGCGGGCGCGCCG765960.15028882861673845No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG762370.14958443557436799No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT753250.1477950025530814No Hit
GTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGAT738160.14483419725799213No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA736090.14442804305250276No Hit
CGCCCGTGGCCGCGGCGCCGGGGTTCGCGTGTCCCCGGCGGCGACCCGCG718970.14106893194236833No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT712530.1398053410808458No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA697160.13678959705264684No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA653620.12824662405409237No Hit
CGGCCCCGGTCGCGGTCCCCCGTCCCGGGCGGGGGCGGGCGCGCCGGCCG648880.12731658978645002No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTG639300.1254369002750547No Hit
CGGCGTCCGGCCGACCGCCGCTCCCGCGCCCCTCCTCCTCCCCGCCGCCC609490.11958788729648534No Hit
CCGGCGTCCGGCCGACCGCCGCTCCCGCGCCCCTCCTCCTCCCCGCCGCC609480.11958592519887429No Hit
CGGCGGGCGGCGGGGAGCGGTCCCCGGCCGCGGCCCCGACGGCGTGGGTG602990.11831252384929647No Hit
CAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCT601630.11804567857419232No Hit
CCCGTGGCCGCGGCGCCGGGGTTCGCGTGTCCCCGGCGGCGACCCGCGGG564150.1106917367279401No Hit
TGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTA553580.10861779955304986No Hit
TCGGCCCCGGTCGCGGTCCCCCGTCCCGGGCGGGGGCGGGCGCGCCGGCC553460.10859425438171716No Hit
CGGGCGGCGGGGAGCGGTCCCCGGCCGCGGCCCCGACGGCGTGGGTGTCG547720.1074680103529688No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT543670.10667336082048957No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG533860.10474854306403988No Hit
CGCGTGTCCCCGGCGGCGACCCGCGGGACGCCGCGGTGTCGTCCGCCGTC529770.10394604514111641No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG517590.10155621025084555No Hit
CAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCT516670.10137569727062805No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)