FastQCFastQC Report
Thu 6 Oct 2022
EGAF00005733414

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005733414
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37189300
Sequences flagged as poor quality0
Sequence length150
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGCCTCGGTGTAGATCT473010.1271898099722232Illumina Single End PCR Primer 1 (96% over 32bp)
TTTTGAAGACGTTTAGGAGGAAGGGTGAGTTTTCTGTTGAGAAACTCAAT467490.12570551206933178No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)