FastQCFastQC Report
Sat 11 May 2024
EGAF00005733654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005733654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174295504
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCAGC6701474138.44892120682585No Hit
GTCAGCTA3885027522.289889359395065No Hit
AATGTTAG3398453819.498229856806862No Hit
CGATAGCT3284898818.846721370391744No Hit
AATGTTAA2311300.13260812510688744No Hit
AATGGTAG2067310.11860948518786806No Hit
TCGCCAGA1912440.10972400068334522No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)