FastQCFastQC Report
Sun 5 May 2024
EGAF00005733661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005733661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162592747
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGACGCCC5429682333.394369676280824No Hit
ACCGTATG3834161723.581382138774No Hit
CTGACTGA3722355522.893736459228407No Hit
GATTAGAT3027864118.622381107811652No Hit
GAGTAGAT9134350.5617932022515125No Hit
TGACGCCA3085950.18979628900666767No Hit
ACCGTATA2284390.14049765700803368No Hit
GCTTAGAT1657580.10194673689841774No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)