FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005733664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005733664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41519360
Sequences flagged as poor quality0
Sequence length93
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTG84400.019.343645
TCGCTAT38500.019.3364451
GTATAGG110200.015.5005711
ATGCCGT304900.015.34151586-87
TATACTG220350.014.9956185
CCTATAC114250.014.3657353
GTATTAG151450.013.9048181
CCCTACG53050.013.7504092
CTAATAC162050.013.7455013
CCGGGTT123200.013.7450454
TGCCGTC279150.013.66050886-87
GTATATA356500.013.6592971
TAGCGCA247450.013.5989854
GTATAGA142150.013.5590551
GTGTAGG132850.013.280031
TACACTG235300.013.2678245
ATAGCGC259850.013.0001733
TACACCG30950.012.906845
GTACTAG77900.012.8293051
TAATACT272900.012.6967214
Produced by FastQC (version 0.11.5)