FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005733665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005733665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41519360
Sequences flagged as poor quality0
Sequence length93
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTAT37700.018.4122891
CGGGTTG82400.017.4288775
GTATAGG110400.015.4222431
TAGCGCA238900.015.1216294
TCTCGGG174750.014.82204686-87
CTCGGTG285900.014.63539786-87
ATAGCGC250600.014.4677353
CCCTACG56450.014.3412222
CATAGCG275500.014.3292292
TATACTG213200.014.145845
GTATTAG151300.014.0521261
TACGTTC55000.013.8470765
CGCCGTA27650.013.76437486-87
AGCGCAC266700.013.5926685
TCGGTGG218000.013.4676286-87
TACACTG230100.013.2960125
GCGCACT270650.013.21066
GTATAGA142450.013.1782581
CTACGTT62600.013.1408694
GTATATA372800.013.0806471
Produced by FastQC (version 0.11.5)