FastQCFastQC Report
Sat 11 May 2024
EGAF00005733701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005733701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172223186
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCCAGC6618784938.43143919077191No Hit
GTCAGCTA3845274822.327277118192438No Hit
AATGTTAG3348762919.44432092900662No Hit
CGATAGCT3245157618.84274513421207No Hit
AATGTTAA2275310.1321140348663623No Hit
AATGGTAG2068900.12012900516194143No Hit
TCGCCAGA1875820.10891797112614093No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)