FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005733723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005733723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91238066
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCGTTCGTTATCGGAATTAACCA998710.10946198706140921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGAAC236550.017.6299781
GGGAATG254350.017.5808411
GGGACCT326500.017.4762041
ATGCGAT240250.017.4126321
GACTAAC224550.017.4085831
GCGCGAT222700.017.314961
ACGGAGA248250.017.2547441
TGCCAAA283050.017.2093471
GAATGAA150250.017.0628091
AGATATG279450.016.916548
ACGGGCT451750.016.9053781
GGCGACT272650.016.6468261
CGGACGT296100.016.5894931
CACGAAA274200.016.5658428
CTCGAAA163050.016.5460171
CACCGAA151500.016.5257028
AGCTGAA197050.016.5213748
ACGAGGA197350.016.4956281
GATCGCG250400.016.4789331
GACCAAT243900.016.4296861
Produced by FastQC (version 0.11.5)