FastQCFastQC Report
Sat 25 May 2024
EGAF00005733735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005733735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166820771
Total Bases15.1 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT75610454.532436191653856Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16158080.9685892172264328Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7623530.4569892558523183Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5369330.32186219784345677No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2716190.16282085160726179Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2289100.13721912363059394Clontech SMART CDS Primer II A (100% over 22bp)
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC1982080.11881494061671734No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1808320.10839897149258469Clontech SMART CDS Primer II A (100% over 21bp)
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGG1796740.10770481332927061No Hit
CCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCAC1689080.101251180525955No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)