FastQCFastQC Report
Sat 25 May 2024
EGAF00005733766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005733766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198985261
Total Bases18.1 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT146068637.340675850358585Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT31292671.5726124559547152Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT14660030.7367394914741952Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6018020.3024354653081567No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5318600.267286128292688Clontech SMART CDS Primer II A (100% over 23bp)
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4441500.22320748670927945Clontech SMART CDS Primer II A (100% over 22bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3596660.18075007073011304Clontech SMART CDS Primer II A (100% over 21bp)
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGG2581350.12972568857750724No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC2417030.12146779052142963No Hit
CCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCAC2416170.12142457123997742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)