FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005733796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005733796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101453848
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT18773631.8504601225179749No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT2549090.2512561179542446No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1596190.1573316371400718No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1311810.12930115770473288No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT2519700.069.9439241
AGCAGTG2723600.064.799152
GTATCAA2785950.063.157269
TGGTATC2846800.061.766857
GGTATCA2866350.061.3660558
GTGGTAT3041750.057.8458486
CAGTGGT3062450.057.601584
AGTGGTA3232000.054.627155
GCAGTGG3605100.049.1858563
ATCAACG2767150.034.09071410-11
CAACGCA2833100.033.3263912-13
AGAGTAC2854100.032.8243118-19
ACGCAGA2938550.032.1449814-15
TCAACGC2809750.031.36861412-13
GCAGAGT3089450.030.48635516-17
AACGCAG2937800.029.99633414-15
TATCAAC2940450.029.99165510-11
GTACTTT2890100.029.97957822-23
CAGAGTA3024800.029.04987518-19
CGCAGAG3106800.028.39629616-17
Produced by FastQC (version 0.11.5)