FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005733797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005733797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100494305
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACCTACTAAGAACGGCCATGCACC1183680.1177857790050889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGAA239050.017.7530141
CCACCTA635850.017.6769141
ACTGAAC249700.017.4368611
GTAACTG361800.017.4218241
GATGAAA195450.017.2722661
TGACTAG305650.017.2158451
TGACGGC327500.017.1096611
GCGCGAT207200.017.0610351
GACCAAT252900.017.0222531
CGGAGCT289000.017.0194821
TTGACTT472550.017.0166231
GACTAAC198650.016.9889931
GGGAATG232450.016.9736141
GGAACTT308850.016.9013291
CCGGGAT261350.016.8932611
CGACCTT403350.016.8483431
GGGACCT259050.016.6954271
CAATGGA360150.016.662868
CTCGAAA180800.016.6617721
AGACTAG194400.016.617968
Produced by FastQC (version 0.11.5)