FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734763

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734763
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27198661
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG103250.012.0992461
GTCTCGA105400.010.27802933
TCGAACT79100.09.98852536
ACGGGGT86250.09.6799325
CGAACTC79300.09.57181437
CTCGAAC81500.09.56703235
GAGACGG129950.09.5306252
TCTCGAA90400.09.19740834
GGTCTCG118100.09.14350832
AGAGCGA70600.09.0428811
AGACGGG104150.08.9102983
CGGGGTT95200.08.84242256
GACGGGG104900.08.4192324
GGGTTTC267250.08.4163458
AGAGATG290700.07.989321
GCTGGTC243550.07.94646529
AGAGCGC37050.07.917181
GTTGGCC177850.07.758679420
GTTTCAC265100.07.71682510
GGGGTTT247100.07.5669247
Produced by FastQC (version 0.11.5)