FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26303700
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG89500.012.9933031
GTCTCGA99050.010.83233433
GAGACGG103300.010.6206312
TCGAACT70550.010.31867336
CGGGGTT73300.010.2607366
AGACGGG85600.010.2370013
CTCGAAC70150.010.22959335
ACGGGGT72750.010.1485965
CGAACTC66350.010.08788237
CTCGATC41250.09.86917335
GGTCTCG105950.09.83383532
GACGGGG78600.09.7881944
TCTCGAT47200.09.42897834
CTCGTGA57300.09.33234449
TCGATCT44200.09.21081636
TCTCGAA79200.09.19124234
AGAGCGA56500.09.1612661
TCGTGAT59850.09.10806950
GGGTTTC243800.09.0278968
CGTTGGA76950.09.01204567
Produced by FastQC (version 0.11.5)