FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38446448
Sequences flagged as poor quality0
Sequence length126
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT411850.10712302993504108No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA554850.061.0443271
TCAACGC643150.051.551014
CAACGCA647950.051.1691175
ATCAACG661700.049.8428843
ACGCAGA733850.045.122347
AACGCAG741350.044.803446
TATCAAC756450.043.98842
CGCAGAG785450.042.188598
GGTATCA266500.038.119061
GCAGAGT959800.034.612459
AGTACGG522650.034.0600412-13
AGAGTAC824150.033.1713910-11
TACGGGG373700.029.98306814-15
GTACGGG540500.026.50801314-15
GAGTACG542700.026.4171412-13
CAGAGTA930100.024.0418749
TACGGGA199450.023.14798414-15
GTATATA256950.017.862431
AGTACTT545050.017.70061112-13
ATAATAC224100.016.438613
Produced by FastQC (version 0.11.5)