FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005734773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82148494
Sequences flagged as poor quality0
Sequence length126
%GC46

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT841980.10249487957746371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1164500.055.7550131
TCAACGC1262050.049.6060834
ATCAACG1292800.048.48653
CAACGCA1293900.048.4305465
GGTATCA604300.046.886321
AACGCAG1404900.044.7321436
ACGCAGA1457000.043.0416647
TATCAAC1505350.042.11922
CGAGACG144400.040.555992120
CGCAGAG1587700.039.5475858
GCAGAGT1938800.032.5405739
AGAGTAC1634950.031.11709610-11
AGTACGG1088400.030.85410512-13
TACGGGG905750.027.36268614-15
GAGTACG1124550.026.86136612-13
TGCCGTT220100.026.66191120
GTACGGG1160200.025.96127314-15
CAGAGTA1828200.024.79731410-11
TCGTATC135700.024.584839120
CTCCGAC347850.023.804523120
Produced by FastQC (version 0.11.5)