FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005734774

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734774
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82148494
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA893950.046.1676941
TCAACGC856500.045.4482574
CAACGCA892950.043.607055
ATCAACG897050.043.51273
AACGCAG990000.039.4291466
ACGCAGA999650.039.0135087
TATCAAC1117700.035.7689172
GGTATCA521900.034.719531
CGCAGAG1132450.034.4670528
AGTACGG1057000.030.76051512-13
GACGACC362550.029.124815120
AGAGTAC1161250.028.4742410-11
TACGGGG865300.027.3713514-15
GCAGAGT1452600.027.0989029
GAGTACG1098100.025.59754212-13
GTACGGG1113150.025.1538114-15
CAGAGTA1350950.021.4347369
ACGGGGG469700.018.31124316-17
GTATATA463250.018.015291
TACGGGA360350.017.09780114-15
Produced by FastQC (version 0.11.5)