FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734775

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734775
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42233565
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT487920.11552896375193523No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA628500.059.9607621
CAACGCA700500.052.9920045
TCAACGC702550.052.888624
ATCAACG720650.051.268863
ACGCAGA791900.046.8151257
AACGCAG801750.046.344746
TATCAAC830200.044.9878232
GGTATCA317050.042.400781
CGCAGAG875300.042.375078
GCAGAGT1032600.036.012889
AGTACGG543450.033.79971712-13
AGAGTAC890850.032.13913710-11
TACGGGG386200.029.17572814-15
GTACGGG563050.028.94683614-15
GAGTACG564700.028.85163112-13
CAGAGTA995600.025.32568410-11
TACGGGA231050.021.3583814-15
GAGTACT495750.017.91172212-13
ACGGGGG202250.017.47290416-17
AGTACTT597200.017.34543412-13
Produced by FastQC (version 0.11.5)