FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26674987
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG86100.011.2623281
GAGACGG99700.09.5850422
ACGTCCG6859.258656E-109.56923632
AGAGCGA52050.09.5469961
ACGGGGT73400.09.3064545
CGGGGTT72850.09.1873566
GACGGGG77450.08.9978624
AGACGGG84200.08.8499763
AGGGACG25350.08.0315441
GAGCGAG46850.07.87922572
CTCGAAC61400.07.754116535
GTCTCGA88950.07.64073933
AGGGAGC117200.07.62600661
TCGAACT61700.07.604910436
AGGGCGC18000.07.4768231
AGTCCCA202750.07.47185851
AGGACGG22300.07.42782071
AGAGCAG281100.07.4271041
CGTTGGA66000.07.42279767
TCGTTGG65100.07.419675466
Produced by FastQC (version 0.11.5)