FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26674987
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG88350.010.3875471
AGGGCGA21400.09.9957611
AGGGGCG31450.09.98294451
AGAGCGA51400.09.4644171
AGGGCCG26400.09.1481241
CGGGGTT75400.08.7839966
AGAGCGG24000.08.769131
AGGGACG25050.08.6770241
GAGACGG100350.08.663032
AGGGGTC79500.08.462631
AGGGGCT129250.08.4084871
GACGGGG76050.08.3910754
TCGAGCG7453.911191E-88.33649448
ACGGGGT72200.08.2653985
AGTCCCG16350.08.2297141
AGGTCCG13050.08.1957581
GAGCGAG45400.08.0544642
AGGACGG22300.08.0451921
AGGGACC87800.08.0162931
AGTCCCA202500.08.0077541
Produced by FastQC (version 0.11.5)