FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005734781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76829906
Sequences flagged as poor quality0
Sequence length126
%GC47

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT802420.10444110135967107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1042750.055.845261
TCAACGC1181400.047.6151544
ATCAACG1214900.046.3861543
CAACGCA1221900.046.0306855
AACGCAG1285400.043.8033146
GGTATCA545700.043.6463361
TATCAAC1388050.041.0413362
ACGCAGA1413650.039.8034637
CGCAGAG1538200.036.59228
AGAGTAC1587900.030.45969810-11
GCAGAGT1883800.029.9904989
TCGTATC132900.029.57285120
AGTACGG1116450.028.00997512-13
TGCCGTT224400.027.809126120
CAGAGTA1754950.024.179369
CTCCGAC363900.023.810143120
TACGGGG1177600.023.50420614-15
CACGAGC365850.022.649963120
TCTCGTT272850.022.211315120
GAGTACG1173300.022.00486212-13
Produced by FastQC (version 0.11.5)