FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005734782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76829906
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA821700.047.5368421
TCAACGC807200.045.6557854
ATCAACG846400.043.6531373
CAACGCA853450.043.0909165
AACGCAG902400.040.9630436
TATCAAC1016950.036.9907952
ACGCAGA1014600.036.3750277
GGTATCA481700.032.6354261
CGCAGAG1141350.032.3428278
AGTACGG1121400.027.12685812-13
AGAGTAC1156150.026.793910-11
GCAGAGT1459050.025.4865679
TACGGGG1174500.022.77306614-15
GAGTACG1173450.022.12342312-13
CAGAGTA1321450.020.428619
GTACGGG1296850.019.93954714-15
GTATATA409150.018.0077021
ACGGGGG759500.016.70278516-17
GTATTAA430800.016.2385221
GTATTAG275650.015.880561
Produced by FastQC (version 0.11.5)