FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40383106
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA515700.051.93541
TCAACGC516950.050.034084
CAACGCA519200.049.955925
ATCAACG540000.047.920593
ACGCAGA573300.045.2627147
AACGCAG614800.042.265966
CGCAGAG643900.040.309238
TATCAAC677250.038.811522
GGTATCA272250.034.4891851
AGTACGG596300.033.95392612-13
GCAGAGT779700.033.4501889
AGAGTAC661500.031.09204710-11
TACGGGG404150.029.59528214-15
GAGTACG609550.028.48619812-13
GTACGGG613650.028.12988314-15
CAGAGTA762500.023.14554210-11
TACGGGA257500.022.34550514-15
GTATATA272000.019.433161
GTATTAG223000.017.5420021
ATAATAC231600.017.0460513
Produced by FastQC (version 0.11.5)