FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005734787

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734787
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85834328
Sequences flagged as poor quality0
Sequence length126
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT881210.10266405301151771No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA1273750.053.88271
TCAACGC1309000.050.4492454
CAACGCA1352250.048.798435
ATCAACG1373850.048.129023
AACGCAG1433700.046.1599126
GGTATCA677800.043.428181
ACGCAGA1529750.043.2376377
TATCAAC1623700.041.218692
CGCAGAG1666800.039.6824728
AGTACGG1101800.032.58582312-13
GCAGAGT2060000.032.2857789
AGAGTAC1726950.030.947710-11
TACGGGG943600.030.48981914-15
TGCCGTT249900.030.422234120
TCGTATC148900.029.498312120
CTCCGAC317550.028.967487120
GTACGGG1126500.028.95323614-15
GAGTACG1166800.027.99144212-13
CAGAGTA1933550.024.69176510-11
ACGGGGG524800.022.61819516-17
Produced by FastQC (version 0.11.5)