FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005734788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85834328
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC925100.046.900934
GTATCAA1023600.045.155811
CAACGCA966750.044.8190735
ATCAACG996200.043.648283
AACGCAG1043700.041.632086
ACGCAGA1087300.039.9200027
TATCAAC1242650.035.7634282
CGCAGAG1229350.035.295668
GGTATCA624100.034.8511161
AGTACGG1113950.031.36123812-13
TACGGGG953550.029.5685914-15
AGAGTAC1249750.028.43082210-11
GTACGGG1142900.027.88156514-15
GCAGAGT1584250.027.5685279
GAGTACG1183650.026.97658312-13
CAGAGTA1461250.022.13679910-11
ACGGGGG532050.022.11261216-17
ACGGGGA496100.018.08269316-17
TACGGGA305400.017.63114-15
GTATAGG245750.017.3739471
Produced by FastQC (version 0.11.5)