FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005734792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005734792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31893199
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGACG136850.011.622491
AGGGACG44000.09.880091
AGGCGCC86400.09.6237941
AGGGGCG65150.09.4794181
AGAGCGA86100.09.4168961
AGGGCGG71300.09.0972751
CGGCTAA69100.08.73720320
GAGACGG166600.08.7176022
AGGGCGC42500.08.6051811
AGGGCCG54300.08.5778211
AGGGCGA37050.08.5672741
ACGGGGT114350.08.5370175
CCGGCTA72350.08.48773619
AGACGGG136050.08.4177653
CGGGGTT118050.08.3571856
GCGAGAC72150.08.2710034
ACCACGC113100.08.23549512
AGGGGGG99050.08.1508781
AGGGGAG258600.08.13851
AGGGGAA275350.08.0067971
Produced by FastQC (version 0.11.5)